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superscript vilo master mix reverse transcriptase enzyme  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript vilo master mix reverse transcriptase enzyme
    Superscript Vilo Master Mix Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+vilo+master+mix+reverse+transcriptase+enzyme/superscript+vilo+master+mix+reverse+transcriptase+enzyme/pm40019931-85-19-26
    Average 90 stars, based on 1 article reviews
    superscript vilo master mix reverse transcriptase enzyme - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Effects of bisphenol A on murine salivary glands and human tumor cell lines.
    Article Snippet: The quality and integrity of mRNA samples were verified by analyzing 1 μL of total mRNA on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Complementary DNA was synthesized from 2.5 μg RNA using the SuperScript VILO Master Mix reverse transcriptase enzyme (ThermoFisher Scientific Inc.). .. The Rotor-Gene Q system (Qiagen Inc.) was employed for the analysis, and the SYBR® Green PCR Master Mix kit (Thermo-Fisher Scientific Inc.) was applied.

    Article Title: Deletion of kinin receptor B2 enhances orthodontic tooth movement and alveolar bone remodeling.
    Article Snippet: Total RNA was extracted from the periodontal ligament of the upper molars and the surrounding alveolar bone from both the control and OTM sides using an RNeasy kit (Qiagen Inc, Valencia, CA), following the manufacturer’s instructions. .. Following RNA extraction, complementary DNA (cDNA) was synthesized from 2,5 μg of RNA via reverse transcription reaction using the SuperScript VILO Master Mix reverse transcriptase enzyme (Thermo Fisher Scientific, MA, USA). .. The target genes analyzed included receptor activator of nuclear factor‐kappa B (RANK), RANKL, osteoprotegerin (OPG), BDKRB1, interleukin‐1β (IL-1β), interleukin‐6 (IL-6) and tumor necrosis factor-alpha (TNF-α). mRNA levels were quantified using a Rotor-Gene Q system (QIAGEN, Hilden, Germany) with the SYBR® Green PCR Master Mix kit (Thermo Fisher Scientific).

    Reverse Transcription:

    Article Title: Effects of bisphenol A on murine salivary glands and human tumor cell lines.
    Article Snippet: The quality and integrity of mRNA samples were verified by analyzing 1 μL of total mRNA on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Complementary DNA was synthesized from 2.5 μg RNA using the SuperScript VILO Master Mix reverse transcriptase enzyme (ThermoFisher Scientific Inc.). .. The Rotor-Gene Q system (Qiagen Inc.) was employed for the analysis, and the SYBR® Green PCR Master Mix kit (Thermo-Fisher Scientific Inc.) was applied.

    Article Title: Deletion of kinin receptor B2 enhances orthodontic tooth movement and alveolar bone remodeling.
    Article Snippet: Total RNA was extracted from the periodontal ligament of the upper molars and the surrounding alveolar bone from both the control and OTM sides using an RNeasy kit (Qiagen Inc, Valencia, CA), following the manufacturer’s instructions. .. Following RNA extraction, complementary DNA (cDNA) was synthesized from 2,5 μg of RNA via reverse transcription reaction using the SuperScript VILO Master Mix reverse transcriptase enzyme (Thermo Fisher Scientific, MA, USA). .. The target genes analyzed included receptor activator of nuclear factor‐kappa B (RANK), RANKL, osteoprotegerin (OPG), BDKRB1, interleukin‐1β (IL-1β), interleukin‐6 (IL-6) and tumor necrosis factor-alpha (TNF-α). mRNA levels were quantified using a Rotor-Gene Q system (QIAGEN, Hilden, Germany) with the SYBR® Green PCR Master Mix kit (Thermo Fisher Scientific).

    RNA Extraction:

    Article Title: Deletion of kinin receptor B2 enhances orthodontic tooth movement and alveolar bone remodeling.
    Article Snippet: Total RNA was extracted from the periodontal ligament of the upper molars and the surrounding alveolar bone from both the control and OTM sides using an RNeasy kit (Qiagen Inc, Valencia, CA), following the manufacturer’s instructions. .. Following RNA extraction, complementary DNA (cDNA) was synthesized from 2,5 μg of RNA via reverse transcription reaction using the SuperScript VILO Master Mix reverse transcriptase enzyme (Thermo Fisher Scientific, MA, USA). .. The target genes analyzed included receptor activator of nuclear factor‐kappa B (RANK), RANKL, osteoprotegerin (OPG), BDKRB1, interleukin‐1β (IL-1β), interleukin‐6 (IL-6) and tumor necrosis factor-alpha (TNF-α). mRNA levels were quantified using a Rotor-Gene Q system (QIAGEN, Hilden, Germany) with the SYBR® Green PCR Master Mix kit (Thermo Fisher Scientific).



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